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low salt ip buffer  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology low salt ip buffer
    Low Salt Ip Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+salt+ip+buffer/Tris+base/10__1128_slash_mcb__01372___13-56-7-39
    Average 94 stars, based on 322 article reviews
    low salt ip buffer - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: Residual Complexes Containing SMARCA2 (BRM) Underlie the Oncogenic Drive of SMARCA4 ( BRG1 ) Mutation
    Article Snippet: Nuclear extracts for immunoprecipitation were prepared using the NE-PER nuclear and cytoplasmic extraction kit (78833; Thermo Scientific). .. Nuclear extracts (125 g) were incubated in low-salt IP buffer (50 mM Tris-HCl [pH 7.5], 1 mM EDTA, 10% glycerol, 100 mM NaCl, 0.05% Tween 20, and protease inhibitors; the total volume was 300 l) with antibodies (SMARCC1, 9746; Santa Cruz; SMARCA4, 17796; Santa Cruz; IgG, 2028; Santa Cruz) overnight at 4°C. .. Protein G Dynabeads (40 l; 10004D; Life Technologies) were added and incubated at 4°C for 3.5 h. Beads were then washed twice with low-salt IP buffer and once with highsalt IP buffer (50 mM Tris-HCl [pH 7.5], 1 mM EDTA, 10% glycerol, 250 mM NaCl, 0.05% Tween 20).



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